Infanta Isabella Clara Eugenia was born on 12 August 1566, in Segovia, Spain. She was the daughter of Élisabeth de Valois, Queen Consort of Spain (2 April 1545 - 3 October 1568) and Felipe II, King of Spain (21 May 1527 - 13 September 1598). Her maternal grandparents were Catherine de' Medici (13 April 1519 - 5 January 1589) and Henri II (31 March 1519 - 10 July 1559). Her paternal grandparents were Isabella of Portugal and Charles V, Holy Roman Emperor. Isabella's parents were married in 1599. She had a younger sister, Infanta Catalina Micaela of Spain (10 October 1567 - 6 November 1597). Isabella's mother was her father's third wife. Felipe II married first, Maria Manuela, Princess of Portugal (15 October 1527 - 12 August 1545). Maria died after she gave birth to a son, Don Carlos of Spain (8 July 1545 - 24 July 1568). His second wife was Mary I, Queen of England (18 February 1516 - 17 November 1558). Her mother, Élisabeth died during childbirth along with her newborn son, on 3 October 1568. Felipe II married his fourth wife, Anna of Austria (1 November 1549 - 26 October 1580) on 4 May 1570, in Prague. Anna and Felipe II had five children: Ferdinand, Prince of Asturias (4 December 1571 - 18 October 1578), Carlos Lorenzo (12 August 1573 - 30 June 1575), Diego, Prince of Asturias (15 August 1575 - 21 November 1582), Felipe III, King of Spain (3 April 1578 - 31 March 1621) and Maria (14 February 1580 - 5 August 1583). Anna died on 26 October 1580. Her father, Felipe II died on 13 September 1598, in Madrid, Spain. Isabella married Archduke Albert of Austria (15 November 1559 - 13 July 1621) on 18 April 1599, in Valencia. He was the son of Maria of Spain, Holy Roman Empress (21 June 1528 - 26 February 1603) and Maximilian II, Holy Roman Emperor. From 1601, the couple ruled the Spanish Netherlands together.
The Children of Isabella Clara Eugenia of Spain and Albert of Austria:
Archduke Philip of Austria (21 October 1605-)
Archduke Albert of Austria (27 January 1607-)
Archduchess Anna Mauritia of Austria
Her husband, Albert of Austria died on 13 July 1621. In 1609 and 1625, she was painted by the artist, Peter Paul Rubens (1577-1640). Isabella Clara Eugenia of Spain died aged 67, on 1 December 1633, in Brussels, Brabant.

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